2012/09/07 | Royal Wootton FC | | 3-1 | | Highworth Town | | | | |
| Worksop Town | | 0-2 | | Frickley Athletic | | | | |
2012/09/08 | Cinderford Town | | 1-1 | | Merthyr Town | | | | |
| Hungerford Town | | 1-1 | | Banbury Utd | | | | |
| Dunstable Town | | 2-2 | | Ashford Town (MIDDX) | | | | |
| Hemel Hempstead | | 2-5 | | Walthamstow FC | | | | |
| Barton Rovers | | 0-1 | | Arlesey Town | | | | |
| North Leigh | | 6-1 | | Wantage Town | | | | |
| Kempston Rovers | | 0-3 | | Hampton & Richmond | | | | |
| Littlehampton | | 0-1 | | Eastbourne Town | | | | |
| Faversham Town | | 2-2 | | Margate FC | | | | |
| Badshot Lea | | 3-2 | | Folkestone | | | | |
| Slough Town | | 4-1 | | Lingfield FC | | | | |
| VCD Athletic | | 0-1 | | Horsham FC | | | | |
| Chalfont St Peter | | 3-2 | | Newport Pagnell | | | | |
| Marlow FC | | 1-3 | | Thatcham Town | | | | |
| Wealdstone FC | | 2-0 | | Biggleswade Town | | | | |
| Enfield Town | | 2-0 | | St. Margaretsbury | | | | |
| Harrow Borough | | 1-3 | | St. Albans | | | | |
| Chesham United | | 0-1 | | Northwood FC | | | | |
| Aveley FC | | 6-0 | | Hadleigh United | | | | |
| Grays Athletic | | 2-1 | | Norwich United | | | | |
| Waltham Abbey | | 1-0 | | Bedford Town | | | | |
| Hendon | | 1-1 | | Potters Bar Town | | | | |
| Wingate & Finchley | | 1-1 | | Royston Town | | | | |
| Leatherhead | | 3-1 | | Tooting & Mitcham Utd | | | | |
| Colney Heath | | 1-2 | | Harefield United | | | | |
| Ware FC | | 0-5 | | Hitchin Town | | | | |
| Uxbridge | | 3-4 | | Berkhamsted Town | | | | |
| Bishop´s Cleeve | | 6-0 | | Bracknell Town | | | | |
| Beckenham Town | | 1-7 | | Metropolitan Police | | | | |
| Bideford | | 2-2 | | Bodmin Town | | | | |
| Clevedon Town | | 5-0 | | Sherborne Town | | | | |
| Gillingham Town | | 0-0 | | Taunton Town | | | | |
| Plymouth Parkway | | 1-1 | | Buckland Athletic | | | | |
| Sholing | | 4-0 | | Melksham Town | | | | |
| Chippenham Town | | 3-1 | | Mangotsfield United | | | | |
| Bashley FC | | 1-1 | | Gosport Borough | | | | |
| Windsor FC | | 0-1 | | Didcot Town | | | | |
| Dunston UTS | | 3-3 | | Kendal Town | | | | |
| Soham Town | | 0-2 | | Maldon & Tiptree | | | | |
| Hastings United | | 3-1 | | Chatham Town | | | | |
| Larkhall Athletic | | 0-0 | | Frome Town FC | | | | |
| Swindon Supermarine | | 0-6 | | AFC Totton | | | | |
| Winchester City | | 1-2 | | Yate Town | | | | |
| Guildford City | | 0-3 | | Kingstonian | | | | |
| Whitehawk | | 5-0 | | Sittingbourne FC | | | | |
| Chipstead | | 0-4 | | Maidstone United | | | | |
| South Park FC | | 1-1 | | Walton & Hersham | | | | |
| East Thurrock | | 2-0 | | Witham Town | | | | |
| Thamesmead Town | | 2-0 | | Worthing | | | | |
| Pagham FC | | 0-0 | | Carshalton Athletic | | | | |
| Blackfield & Langley | | 4-0 | | Almondsbury FC | | | | |
| Totton & Eling | | 2-2 | | Weymouth | | | | |
| Newport Isle of Wight | | 2-0 | | Horndean FC | | | | |
| Fareham Town | | 2-1 | | Christchurch FC | | | | |
| Bognor Regis Town | | 4-0 | | Epsom | | | | |
| Redhill FC | | 1-3 | | Lewes | | | | |
| Bugbrooke St Michaels | | 1-4 | | Dereham Town | | | | |
| Stocksbridge Park | | 0-3 | | Marine AFC | | | | |
| Runcorn Town | | 0-4 | | Trafford | | | | |
| Bootle | | 1-2 | | Bamber Bridge | | | | |
| Blyth Spartans | | 1-0 | | Garforth Town | | | | |
| Skelmersdale United | | 1-0 | | Clitheroe FC | | | | |
| Curzon Ashton | | 8-1 | | Maltby Main | | | | |
| AFC Fylde | | 3-1 | | Ramsbottom United | | | | |
| Quorn | | 1-2 | | Buxton | | | | |
| Leicester Nirvana | | 2-2 | | Gresley Rovers | | | | |
| Holbeach United | | 1-2 | | Stamford | | | | |
| Matlock Town | | 2-2 | | Belper Town | | | | |
| Retford United | | 1-2 | | New Mills | | | | |
| Chorley | | 2-0 | | Nantwich Town | | | | |
| Lancaster City | | 0-4 | | Salford City | | | | |
| Farsley Celtic | | 1-1 | | Ossett Albion | | | | |
| Ossett Town | | 1-1 | | Whitby Town | | | | |
| Durham City | | 1-3 | | Shildon AFC | | | | |
| Tadcaster Albion | | 3-0 | | Holker Old Boys | | | | |
| Spennymoor Town | | 2-0 | | South Shields | | | | |
| Bedlington Terriers | | 0-1 | | Bishop Auckland | | | | |
| Ashington AFC | | 2-3 | | West Auckland Town | | | | |
| Burscough | | 2-4 | | Witton Albion | | | | |
| Warrington Town | | 1-0 | | Maine Road | | | | |
| Abbey Hey | | 2-1 | | Atherton Collieries | | | | |
| Eccleshill United | | 0-2 | | North Ferriby United | | | | |
| Rainworth Miners Welfare | | 2-2 | | Carlton Town | | | | |
| Loughborough Dynamo | | 2-2 | | Grantham Town | | | | |
| Heybridge Swifts | | 4-1 | | AFC Sudbury | | | | |
| Basildon Utd | | 0-3 | | Thurrock | | | | |
| Tilbury FC | | 0-2 | | Lowestoft Town | | | | |
| St Neots Town | | 5-0 | | Peterborough Northern Star | | | | |
| Daventry Town | | 1-1 | | King´s Lynn | | | | |
| Bury Town | | 3-0 | | Canvey Island | | | | |
| Wroxham | | 0-5 | | Brentwood Town | | | | |
| Sutton Coldfield Town | | 0-3 | | Gornal Athletic | | | | |
| Wisbech Town | | 0-0 | | Kettering Town | | | | |
| Barwell FC | | 3-0 | | AFC Wulfrunians | | | | |
| Concord Rangers | | 1-0 | | Needham Market | | | | |
| Leiston | | 5-1 | | Southend Manor | | | | |
| Coalville Town | | 0-1 | | Ilkeston FC | | | | |
| Cambridge City | | 7-0 | | Huntingdon Town | | | | |
| Redditch United | | 0-3 | | Hednesford Town | | | | |
| Leek Town | | 5-0 | | Bewdley Town | | | | |
| Chasetown | | 1-1 | | Market Drayton Town | | | | |
| Rugby Town FC | | 1-1 | | Bedworth United | | | | |
| Dudley Town | | 0-1 | | Nuneaton Griff | | | | |
| Prescot Cables | | 1-3 | | Ashton United | | | | |
| Norton United | | 2-5 | | Westfields FC | | | | |
| Eastwood | | 1-1 | | Blaby & Whetstone | | | | |
| Stourport Swifts | | 1-1 | | Stafford Rangers | | | | |
| Wolverhampton Casuals | | 1-1 | | Tividale FC | | | | |
| Bridgnorth Town | | 3-1 | | Rushall Olympic | | | | |
| Leamington | | 2-2 | | Stourbridge FC | | | | |
2012/09/09 | London Lions | | 2-4 | | AFC Dunstable | | | | |
| Godalming Town | | 2-2 | | Dulwich Hamlet | | | | |
| FC United of Manchester | | 5-0 | | Cammell Laird | | | | |
| Greenwich Borough | | 0-1 | | Cray Wanderers | | | | |