| 2020/09/16 | Reinmeer Aomori |  | 2-1 |  | Sapporo University | | | | |
| | Tonan Maebashi |  | 2-3 |  | Vonds Ichihara | | | | |
| | Iwaki |  | 4-0 |  | Oyama SC | | | | |
| | Kanazawa Seiryo University |  | 1-2 |  | Toyama Shinjo | | | | |
| | Ococias Kyoto |  | 0-1 |  | Nara | | | | |
| | Tokyo Musashino |  | 3-3
(5-4
Pen.) |  | Tochigi City | | | | |
| | National Institute of Fitness |  | 1-0 |  | Okinawa SV | | | | |
| | Cento Cuore Harima |  | 1-1
(2-4
Pen.) |  | MIO Biwako Shiga | | | | |
| | Verspah Oita |  | 2-2
(5-4
Pen.) |  | Tegevajaro Miyazaki | | | | |
| | Toin University |  | 4-2 |  | Yamanashi Gakuin University | | | | |
| | University of Tsukuba |  | 2-0 |  | Tokyo Int. University | | | | |
| | Maruyasu Okazaki |  | 3-2 |  | Suzuka Point Getters | | | | |
| | Yonago Genki |  | 0-5 |  | Kagura Shimane | | | | |
| | EV Internacional |  | 0-4 |  | MD Nagasaki | | | | |
| | Kumamoto Teachers Club |  | 0-0
(1-3
Pen.) |  | Fukuoka University | | | | |
| | Sony Sendai |  | 3-0 |  | Saruta Kogyo | | | | |
24/04 - 23:00
25/04 - 16:00
25/04 - 19:00
25/04 - 21:00
