2021/04/16 | Chelsea | | 5-0 | | London City Lionesses | | | | |
2021/04/17 | Manchester City | | 8-0 | | Aston Villa | | | | |
2021/04/18 | Birmingham City | | 5-1 | | Coventry United | | | | |
| West Ham | | 11-0 | | Chichester & Selsey Ladies | | | | |
| Crystal Palace | | 3-0 | | London Bees | | | | |
| Leicester City | | 1-0 | | Liverpool | | | | |
| Middlesbrough | | 0-9 | | Sheffield United | | | | |
| Brighton & Hove Albion | | 1-0 | | Bristol City | | | | |
| Lewes | | 1-2 | | Southampton | | | | |
| Everton | | 2-1 | | Durham Women | | | | |
| Burnley | | 0-6 | | Manchester United | | | | |
| Huddersfield Town | | 3-2 | | Derby County | | | | |
| Reading | | 2-3 | | Tottenham | | | | |
| Arsenal | | 10-0 | | Gillingham | | | | |
| Oxford United | | 1-2 | | Charlton Athletic | | | | |
| Southampton | | 2-5 | | Blackburn Rovers | | | | |