2024/08/21 | Horovicko |  | ANU |  | FK Robstav | | | | |
2024/08/13 | Slaný |  | 0-5 |  | SK Sokol Brozany | | | | |
| Slaný |  | 0-5 |  | SK Sokol Brozany | | | | |
2024/08/20 | HFK Olomouc |  | 0-2 |  | MFK Vyskov | | | | |
| Rokycany |  | 1-6 |  | FK Viagem Pribram | | | | |
| FK Admira |  | 3-2 |  | Sokol Hostoun | | | | |
| Arsenal Ceska Lipa |  | 2-0 |  | Sparta Kolín | | | | |
| Kurim |  | 1-0 |  | Start Brno | | | | |
| Rokycany |  | 1-6 |  | FK Viagem Pribram | | | | |
| HFK Olomouc |  | 0-2 |  | MFK Vyskov | | | | |
| SK Aritma Prague |  | 3-2 |  | Sokol Hostoun | | | | |
| Kurim |  | 1-0 |  | Start Brno | | | | |
2024/08/21 | FK Pencin-Turnov |  | 0-4 |  | SK Zápy | | | | |
| FC Vítkovice |  | 2-0 |  | Frýdek-Místek | | | | |
| Spartak Sobeslav |  | 1-3 |  | Povltava FA | | | | |
| Olympie Brezová |  | 1-3 |  | Petrin Plzen | | | | |
| Olympie Brezová |  | 1-3 |  | Petrin Plzen | | | | |
| Marianske Lazne |  | 0-4 |  | FK Baník Most - Sous | | | | |
| Spartak Sobeslav |  | 1-3 |  | Povltava FA | | | | |
| Horovicko |  | ANU |  | FK Robstav | | | | |
| Jiskra Rýmasov |  | 0-6 |  | Kromeriz | | | | |
| SK Hrebec |  | 1-3 |  | FK Kladno | | | | |
| Zdirec Nad Doubravou |  | 0-4 |  | SK Lisen | | | | |
| Slovan Velvary |  | 2-2 (0-1 Pen.) |  | Motorlet Prague | | | | |
| FK Pencin-Turnov |  | 0-4 |  | SK Zápy | | | | |
| SK Cesky Brod |  | 1-4 |  | FK Admira | | | | |
| Rapid Psary |  | 2-10 |  | Táborsko | | | | |
| Zdirec Nad Doubravou |  | 0-4 |  | SK Lisen | | | | |
| Beskyd Frenstat |  | 0-0 |  | Zlínsko | | | | |
| FK Horni Redice |  | 0-2 |  | TJ Sokol Zivanice | | | | |
| SK Cesky Brod |  | 1-4 |  | FK Admira | | | | |
| FK Kozlovice |  | 0-0 |  | Uhersky Brod | | | | |
| SK Hrebec |  | 1-3 |  | SK Kladno | | | | |
| Slavoj Kolovec |  | 0-7 |  | Jiskra Domazlice | | | | |
| Meteor Praha |  | 0-5 |  | Ústí nad Labem | | | | |
| TJ Ligmet Milin |  | 1-1 |  | FC Sellier & Bellot Vlasim | | | | |
| Velke Hamry |  | 0-3 |  | Benatky | | | | |
| FK Dobrovice |  | 1-5 |  | Viktoria Zizkov | | | | |
| Kvasice |  | 1-3 |  | FC Strani | | | | |
| Marianske Lazne |  | 0-4 |  | FK Baník Most - Sous | | | | |
| Velke Hamry |  | 0-3 |  | Benatky | | | | |
| Kvasice |  | 1-3 |  | FC Strani | | | | |
| FK Horni Redice |  | 0-2 |  | TJ Sokol Zivanice | | | | |
| FK Kozlovice |  | 0-0 (8-9 Pen.) |  | Uhersky Brod | | | | |
| Jiskra Rýmasov |  | 0-6 |  | Kromeriz | | | | |
| Kraluv Dvur |  | 1-1 (3-4 Pen.) |  | FK Loko Vltavín | | | | |
| Slavoj Kolovec |  | 0-7 |  | Jiskra Domazlice | | | | |
| FK Meteor Praha VIII |  | 0-5 |  | Ústí nad Labem | | | | |
| FC Vítkovice |  | 2-0 |  | Frýdek-Místek | | | | |
| Beskyd Frenstat |  | 0-0 (5-3 Pen.) |  | Zlínsko | | | | |
| FK Prepere |  | 2-3 |  | FK Varnsdorf | | | | |
| Rapid Psary |  | 2-10 |  | Táborsko | | | | |
| FK Dobrovice |  | 1-5 |  | Viktoria Zizkov | | | | |
| TJ Ligmet Milin |  | 1-1 (4-3 Pen.) |  | FC Sellier & Bellot Vlasim | | | | |
2024/08/27 | Nad Sazavou |  | 2-2 (8-7 Pen.) |  | Jihlava | | | | |
| Nad Sazavou |  | 2-2 |  | Jihlava | | | | |
| SK Vysoke Myto |  | 1-0 |  | MFK Chrudim | | | | |
| SK Vysoke Myto |  | 1-0 |  | MFK Chrudim | | | | |
| SK Hranice |  | 1-2 |  | FC Zlín | | | | |
2024/08/28 | MFK Havirov |  | 2-2 (3-5 Pen.) |  | FC Hlucin | | | | |
| Bzenec |  | 3-3 (5-4 Pen.) |  | Hodonin | | | | |
| Sokol Tasovice |  | 2-1 |  | Slovan Rosice | | | | |
| Bílovec |  | 2-2 |  | SK Unicov | | | | |
| Bzenec |  | 3-3 |  | Hodonin | | | | |
| Sokol Tasovice |  | 2-1 |  | Slovan Rosice | | | | |
| MFK Havirov |  | 2-2 |  | FC Hlucin | | | | |
| Bílovec |  | 2-2 (3-2 Pen.) |  | SK Unicov | | | | |
2024/08/29 | Tatran Bohunice |  | 2-6 |  | Zbrojovka Brno | | | | |
2024/09/04 | Slavia Hradec Králové |  | 1-2 |  | Jiskra Usti Orl | | | | |
| SK Benesov |  | 1-3 |  | FC Písek | | | | |
| FC Vratimov |  | 0-4 |  | SFC Opava | | | | |
| FC Vratimov |  | 0-4 |  | SFC Opava | | | | |
| FK Bohumín |  | 0-2 |  | SK Prostejov | | | | |
| FK Bohumín |  | 0-2 |  | SK Prostejov | | | | |
| Slavia Hradec Králové |  | 1-2 |  | Jiskra Usti Orl | | | | |
| SK Benesov |  | 1-3 |  | FC Písek | | | | |
2024/09/11 | Spartak Police n/Metují |  | 2-0 |  | Chlumec Nad Cidlinou | | | | |
| Spartak Police n/Metují |  | 2-0 |  | Chlumec Nad Cidlinou | | | | |
| Karlovy Vary |  | 5-1 |  | FC Chomutov | | | | |