| 2019/08/27 | Dolni Benesov |  | 2-3 |  | SK Prostejov | | | | |
| | Slovan Rosice |  | 1-5 |  | Sigma Olomouc | | | | |
| | Valasské Mezirici |  | 0-5 |  | Trinec | | | | |
| | SFK Vrchovina |  | 0-5 |  | 1. FC Slovácko | | | | |
| 2019/08/28 | Olešnice U Bouzova |  | 0-6 |  | SFC Opava | | | | |
| | Marianske Lazne |  | 2-5 |  | Slovan Liberec | | | | |
| | Sokol Lanzhot |  | 1-3 |  | Baník Ostrava | | | | |
| | FC Písek |  | 1-3 |  | Teplice | | | | |
| | SK Zápy |  | 2-3 |  | Bohemians 1905 | | | | |
| | TJ Stechovice |  | 1-4 |  | Banik Sokolov | | | | |
| | MFK Frýdek-Místek |  | 1-3 |  | MFK Karviná | | | | |
| | Jiskra Usti Orl |  | 0-2 |  | Ceské Budéjovice | | | | |
| | SK Unicov |  | 1-4 |  | FC Fastav Zlín | | | | |
| | Chlumec Nad Cidlinou |  | 2-1 |  | 1.FK Pribram | | | | |
| | Táborsko |  | 4-4
(3-5
Pen.) |  | FK Varnsdorf | | | | |
| | Kraluv Dvur |  | 0-2 |  | Dukla Praha | | | | |
| | Jiskra Domazlice |  | 2-2
(4-3
Pen.) |  | FK Ústí nad Labem | | | | |
| | Odra Petrkovice |  | 3-4
Pro. |  | Zbrojovka Brno | | | | |
| | Motorlet Prague |  | 0-5 |  | FK Slavoj Vysehrad | | | | |
| | Zdirec Nad Doubravou |  | 0-4 |  | SK Lisen | | | | |
| | FK Prepere |  | 0-1 |  | MFK Chrudim | | | | |
| | FK Loko Vltavín |  | 2-1 |  | FC Sellier & Bellot Vlasim | | | | |
| | Slovan Velvary |  | 0-1 |  | Viktoria Zizkov | | | | |
| 2019/09/03 | SK Benesov |  | 2-1 |  | FK Pardubice | | | | |
| 2019/09/04 | FK Admira |  | 1-2 |  | FC Hradec Králové | | | | |
| | Sokol Hostoun |  | 1-6 |  | Jihlava | | | | |
| 2019/09/10 | FC Hlucin |  | 4-2 |  | FC Vítkovice | | | | |
26/04 - 15:45
